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Biographie de Johan Owen De Craene

GENERAL INFORMATION

Date of Birth: Septermber 7, 1973
Place of Birth: Perpignan, France
Country of Citizenship: Belgian
Languages: French and English; Fluent in Dutch

EDUCATION

DOCTORATE IN SCIENCES, FREE UNIVERSITY BRUSSELS (BELGIUM) 2001

 Title of Doctoral Thesis: “Role of the protein kinase Npr1 in regulating the intracellular trafficking of General Amino acid Permease Gap1 in yeast Saccharomyces cerevisiae”.

 M.S. IN CHEMISTRY, FREE UNIVERSITY BRUSSELS (BELGIUM) 1995

RESEARCH EXPERIENCE

 

2006 - PRESENT POST DOCTORAL ASSOCIATE

UNIVERSITE DE STRASBOURG, STRASBOURG, FRANCE
Laboratory of Professor Sylvia Friant

• Characterization of the Proline Rich Domain of Bro1

• Biocomputational study of ENTH, ANTH and VHS containing proteins in collaboration with the laboratory of Olivier Poch, Université Louis Pasteur, Strasbourg.

 

2002 - 2006 POST DOCTORAL ASSOCIATE

 DEPARTMENT OF CELL BIOLOGY, YALE SCHOOL OF MEDICINE, YALE UNIVERSITY, NEW HAVEN, CT 06511
Laboratory of Professor Peter J. Novick

• Study of the role of two exocyst proteins, Sec5 and Exo70 in ER inheritance.

• Identification and characterization of Rtn1 in ER structure.

• Performing a genetic screen for ER inheritance mutants via fluorescent microscopy

 

2001 - 2002 POST DOCTORAL ASSOCIATE

FREE UNIVERSITY BRUSSELS, BRUSSELS, BELGIUM

Laboratory of Professor Bruno André

• Study of the inactivation of the General Amino acid Permease Gap1 by its substrates

• The role of this regulation in the expression of the Asparagine Glutamine Permease Agp1 in yeast.

TEACHING EXPERIENCE

1999 - 2000 Experimental microscopy module (Master1 Students)
2008 – 2011 Supervision of BTS students (First and second year)
2012             Supervisor of ESBS student (First year)

PUBLICATIONS

Les phosphoinositides, des lipides acteurs essentiels du trafic intracellulaire
Bertazzi DL, De Craene JO, Bär S, Sanjuan-Vazquez M, Raess MA, Friant S., Biol Aujourdhui. 2015 Jun 26.

Btn3 regulates the endosomal sorting function of the yeast Ent3 epsin, an adaptor for SNARE proteins.
Morvan J, de Craene JO, Rinaldi B, Addis V, Misslin C, Friant S., J Cell Sci. 2014 Dec 15. pii: jcs.159699.


De Craene JO, Courte F, Rinaldi B, Fitterer C, Herranz MC, Schmitt-Keichinger C, Ritzenthaler C, Friant S.
Study of the Plant COPII Vesicle Coat Subunits by Functional Complementation of Yeast Saccharomyces cerevisiae Mutants. PLoS One. 2014 Feb 25;9(2)

Spiess M, De Craene JO, Michelot A, Rinaldi B, Huber A, Drubin DG, Winsor B, Friant S. Lsb1 is a negative regulator of Las17 dependent actin polymerization involved in endocytosis. PLoS One 2013.

De Craene J.-O., Thompson J., Lecompte O., Ripp R., Poch O. And Friant S. Evolutionary analysis of the ENTH/ANTH/VHS protein superfamily reveals a coevolution between membrane trafficking and metabolism. BMC Genomics. 13 (2012).

De Craene J.-O., Coleman J., Estrada de Martin P., Pypaert M., Anderson S, Yates J.R. 3rd, Ferro-Novick S. and Novick P. 2006. Rtn1p Is Involved in Structuring the Cortical ER. Mol. Biol. Cell. 17 (7):3009-20.

Wiederkehr A, De Craene J.-O., Ferro-Novick S, Novick P. 2004. Functional specialization within a vesicle tethering complex: bypass of a subset of exocyst deletion mutants by Sec1p or Sec4p. J Cell Biol. 167(5):875-87.

De Craene J.-O.*, Soetens O.* and André B. 2001. The Npr1 kinase controls biosynthetic and endocytic sorting of the yeast Gap1 permease. J.Biol.Chem. 276: 43939-43948.

*equal contributors

Soetens O.*, De Craene J.-O.* and André B. 2001. Ubiquitin is required for sorting to the vacuole of the yeast Gap1 permease. J.Biol.Chem. 276: 43949-43957.

*equal contributors

Iraqui I., Vissers S., Bernard F., De Craene J.-O., Boles E., Urrestarazu B. and André B. 1999. Amino acid signalling in Saccharomyces cerevisiae: a permease-like sensor of external amino acids and F-box protein Grr1p are required for transcriptional induction of the AGP1 gene, which encodes a broad-specificity amino acid permease. Mol. Cell. Biol. 19 (2): 989-1001.

Springael J.Y., De Craene J.-O. and André B. 1999. The yeast Npi1/Rsp5 ubiquitin ligase lacking its N-terminal C2 domain is competent for ubiquitination but not for subsequent endocytosis of the Gap1 permease. BBRC 257 (2): 561-566.

 

Courses

June 2009 FEBS/EMBO Advanced Lecture Course in Cellular and Molecular Biology of Membranes.
Cargese, France

PRESENTATIONS

2013 Etude de la plasticité du vivant par évolution synthétique de levure
Vidéo conférence de Johan Owen De Craene

2012 Synthetic Biology Conferences: Use of the yeast Saccharomyces cerevisiae in synthetic biology at ESBS (oral presentation)

2010 Rubicon Workshop: Role of various types of ubiquitin chains in membrane trafficking in Paris, France (poster presentation).

2008 Golgi Meeting: Membrane trafficking in global cellular response in Pavia, Italy (poster presentation).
VIII ème Congrès Levure, Modèle et Outil in La Colle-sur -Loup, France (oral presentation)

2007 European Life Scientist Organization meeting in Dresden, Germany (poster presentation).

2001 Yeast Cell Biology Meeting, Cold Spring Harbor Laboratory, NY (poster presentation).

2000 V ème Congrès Levure, Modèle et Outil in Bruxelles, Belgium (poster presentation).

1999 17th Small Meeting on Yeast Transport and Energetics in Cordoba, Spain (oral presentation)
XIXth International Conference on Yeast Genetics and Molecular Biology in Rimini, Italy (poster presentation).

1998 Réunion de la Société belge de biochimie et de biologie moléculaire in Bruxelles, Belgium (poster presentation).
IV ème Levure, Modèle et outil in Arcachon, France (poster presentation).
Yeast genetics and molecular biology meeting in College Park, Maryland (poster presentation).

1997 Colloque U.L.B.-Lille, l'U.L.B in Bruxelles, Belgium, (poster presentation).
Réunion de la Société belge de biochimie et de biologie moléculaire in Liège ,Belgium (poster presentation).

1996 III ème Congrès Levure, Modèle et Outil in Strasbourg, France (poster presentation).